Since the pioneering work of Hirschfeld. it is known that time-integrated emission (TiEm) of a fluorophore is independent of fluorescence quantum yield and illumination intensity. Practical implementation of this important result for determining exact probe distribution in living cells is often hampered by the presence of autofluorescence. Using kinetic modelling of photobleaching com... https://www.ealisboa.com/top-offer-Birkenstock-Arizona-Eva-Beetroot-Purple-best-save/